Polypeptide synthesis with sucrose gradient fractions of E. coli ribosomes.
نویسندگان
چکیده
* Contribution Number 369 from the McCollum-Pratt Institute and The Department of Biology, The Johns Hopkins University, Baltimore, Maryland. t The following abbreviations are used: DPN, DPNH, and TPN for the oxidized and reduced diand triphosphopyridine nucleotides; RNA for ribonucleic acid; ADP and ATP for the adenosine diand triphosphates; Pi for inorganic phosphate; G-6-P for glucose-6-phosphate; SDPNH for the supernatant solution containing the intermediate which is removed from particles after they have oxidized DPNH in the absence of Pi and ADP; DNP for 2-4 dinitrophenol; and TCA for trichloroacetic acid; Rx for treatment; ADH for alcohol dehydrogenase; and GG for glycylglycine. 1 Pinchot, G. B., J. Biol. Chem., 205, 65 (1953). 2 Ibid., 229, 1 (1957). 3Ibid., 229,25 (1957). 4 Shibko, S., and G. B Pinchot, Arch. Biochem. Biophys., 94, 257 (1961). 5 Pinchot, G. B., Biochem. Biophys. Res. Com., 1, 17 (1959). 6 Shibko, S., and G. B. Pinchot, Arch. Biochem. Biophys., 93, 140 (1961). 7 Pinchot, G. B., these PROCEEDINGS, 46, 929 (1960). 8 Pinchot, G. B., and M. Hormanski, Federation Proc., 21, 54 (1962). 9 Burk, D., and M. Lineweaver, J. Bacteriol., 19, 389 (1930). 10 Lowry, 0. H., N. J. Rosebrough, A. L. Parr, and R. J. Randall, J. Biol. Chem., 193, 265 (1951). 11 Suskind, S. R., personal communications. 12 Smith, I., Chromatographic and Electrophoretic Techniques II (London: William Heinemann, Ltd., 1960). 13 Pabst Laboratories, Circular OR-10, January 1956. 14 Carter, C. E., J. Am. Chem. Soc., 72, 1466 (1950). 15 Kunitz, M., and M. R. McDonald, J. Gen. Physiol., 27,393 (1948). 16 Linnane, A. W., and D. M. Ziegler, Biochim. et Biophys. Acta, 29, 630 (1958). 17 Linnane, A. W., Biochim. et Biophys. Acta, 30, 221 (1955). 18 Wadkins, C. L., and A. L. Lehninger, J. Biol. Chem., 234, 681 (1959). 19 Eisenhardt, R. H., and L. Schachinger, Fed. Proc., 17, 215 (1958). 20 Lehninger, A. L., C. L. Wadkins, C. Cooper, T. M. Devlin, and J. L. Gamble, Science, 128, 3322 (1958). 21 McElroy, W. D., The Harvey Lectures, 51, 240 (1956).
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 48 شماره
صفحات -
تاریخ انتشار 1962